Optimized CUBIC protocol for three-dimensional imaging of chicken embryos at single-cell resolution Articles uri icon

publication date

  • June 2017

start page

  • 2092

end page

  • 2097

issue

  • 11

volume

  • 144

international standard serial number (ISSN)

  • 0950-1991

electronic international standard serial number (EISSN)

  • 1477-9129

abstract

  • The CUBIC tissue-clearing protocol has been optimized to produce translucent immunostained whole chicken embryos and embryo brains. When combined with multispectral light-sheet microscopy, the validated protocol presented here provides a rapid, inexpensive and reliable method for acquiring accurate histological images that preserve three-dimensional structural relationships with single-cell resolution in whole early-stage chicken embryos and in the whole brains of late-stage embryos.

keywords

  • Tissue clearing
    3D imaging
    Chicken embryo
    Light-sheet microscopy