Electronic International Standard Serial Number (EISSN)
1477-9129
abstract
The CUBIC tissue-clearing protocol has been optimized to produce translucent immunostained whole chicken embryos and embryo brains. When combined with multispectral light-sheet microscopy, the validated protocol presented here provides a rapid, inexpensive and reliable method for acquiring accurate histological images that preserve three-dimensional structural relationships with single-cell resolution in whole early-stage chicken embryos and in the whole brains of late-stage embryos.
Classification
subjects
Biology and Biomedicine
keywords
tissue clearing; 3d imaging; chicken embryo; light-sheet microscopy