In-vitro study of the bioactivity and cytotoxicity response of Ti surfaces modified by Nb and Mo diffusion treatments Articles uri icon

publication date

  • February 2018

start page

  • 148

end page

  • 158


  • 335

International Standard Serial Number (ISSN)

  • 0257-8972

Electronic International Standard Serial Number (EISSN)

  • 1879-3347


  • This work focuses on the bioactivity and biological response of modified Ti surfaces produced by powder metallurgy. They are processed by diffusion of two beta-stabilizing elements, Nb and Mo, deposited onto the surface of PM Ti substrates. Moreover, the addition of an activating agent, NH4Cl, to the suspension has been carried out by thermo-reactive diffusion process. The surface modification led to a gradient in composition (Ti-Nb or Ti-Mo) and microstructure (beta / alpha + beta / alpha phases). This work presents the bioactivity results of these Ti-Mo and Ti-Nb surfaces as well as the cell-material response of the Ti-Nb surfaces. The reactivity of the materials was tested through immersion in simulated body fluid considering Ca and P precipitation in order to assess the ability of the materials to induce hydroxyapatite formation. The in-vitro cell response was evaluated by human osteoblast-like cells incubation on the different surfaces for 48 h. The investigation led to positive results in terms of surface bioactivity and an improved cell-material interaction of the PM modified Ti-Nb surfaces compared to the reference Ti material.


  • Materials science and engineering


  • beta-gradient titanium; surface modification; powder technology; bioactivity; cytotoxicity