Looking inside the heart: a see-through view of the vascular tree Articles uri icon

publication date

  • June 2017

start page

  • 3110

end page

  • 3118


  • 6


  • 8

International Standard Serial Number (ISSN)

  • 2156-7085


  • The ability to acquire 3D images of the heart and its vasculature at cellular resolution facilitates a more detailed study of many heart diseases. Here, we describe a novel technique to image in 3D the heart vasculature by combining the CUBIC clearing protocol combined with in vivo administration of fluorescent-labeled lectin. The use of these techniques in combination with Selective Plane Illumination Microscopy (SPIM) made it possible to obtain high resolution 3D images of the cardiac vascular tree. This methodological approach may enhance the visualization of 3D images of the cardiac vasculature remodeling associated with coronary disease.


  • Biology and Biomedicine
  • Chemistry
  • Computer Science
  • Electronics
  • Industrial Engineering
  • Mechanical Engineering
  • Optics
  • Physics


  • microscopy; tissue; three-dimensional microscopy; medical and biological imaging