- OPTICS EXPRESS Journal
- September 2014
Digital Object Identifier (DOI)
International Standard Serial Number (ISSN)
- A new method to obtain the three-dimensional localization of fluorochrome distributions in micrometric samples is presented. It uses a microlens array coupled to the image port of a standard microscope to obtain tomographic data by a filtered back-projection algorithm. Scanning of the microlens array is proposed to obtain a dense data set for reconstruction. Simulation and experimental results are shown and the implications of this approach in fast 3D imaging are discussed.
- Biology and Biomedicine
- three-dimensional microscopy; fluorescence microscopy; tomographic image processing