Plenoptic projection fluorescence tomography Articles uri icon

publication date

  • September 2014

start page

  • 23215

end page

  • 23225

issue

  • 19

volume

  • 22

international standard serial number (ISSN)

  • 1094-4087

abstract

  • A new method to obtain the three-dimensional localization of fluorochrome distributions in micrometric samples is presented. It uses a microlens array coupled to the image port of a standard microscope to obtain tomographic data by a filtered back-projection algorithm. Scanning of the microlens array is proposed to obtain a dense data set for reconstruction. Simulation and experimental results are shown and the implications of this approach in fast 3D imaging are discussed.

keywords

  • three-dimensional microscopy; fluorescence microscopy; tomographic image processing