Laser reflectance measurement for the online monitoring of Chlorella sorokiniana biomass concentration Articles uri icon

publication date

  • February 2017

start page

  • 10

end page

  • 15

volume

  • 243

International Standard Serial Number (ISSN)

  • 0168-1656

Electronic International Standard Serial Number (EISSN)

  • 1873-4863

abstract

  • Fast and reliable methods to determine biomass concentration are necessary to facilitate the large scale production of microalgae. A method for the rapid estimation of Chlorella sorokiniana biomass concentration was developed. The method translates the suspension particle size spectrum gathered though laser reflectance into biomass concentration by means of two machine learning modelling techniques. In each case, the model hyper-parameters were selected applying a simulated annealing algorithm. The results show that dry biomass concentration can be estimated with a very good accuracy (R2 = 0.87). The presented method seems to be suited to perform fast estimations of biomass concentration in suspensions of microalgae cultivated in moderately turbid media with tendency to aggregate.

subjects

  • Biology and Biomedicine
  • Chemistry

keywords

  • bioprocess monitoring; fast estimation dry biomass concentration; on-line monitoring microalgal cultures; cell aggregates; machine learning regression