Single plane illumination microscopy for microfluidic device imaging Articles uri icon

publication date

  • December 2022

start page

  • 1

end page

  • 13


  • 12, 1110


  • 12

International Standard Serial Number (ISSN)

  • 2079-6374


  • Three-dimensional imaging of live processes at a cellular level is a challenging task. It requires high-speed acquisition capabilities, low phototoxicity, and low mechanical disturbances. Three-dimensional imaging in microfluidic devices poses additional challenges as a deep penetration of the light source is required, along with a stationary setting, so the flows are not perturbed. Different types of fluorescence microscopy techniques have been used to address these limitations; particularly, confocal microscopy and light sheet fluorescence microscopy (LSFM). This manuscript proposes a novel architecture of a type of LSFM, single-plane illumination microscopy (SPIM). This custom-made microscope includes two mirror galvanometers to scan the sample vertically and reduce shadowing artifacts while avoiding unnecessary movement. In addition, two electro-tunable lenses fine-tune the focus position and reduce the scattering caused by the microfluidic devices. The microscope has been fully set up and characterized, achieving a resolution of 1.50 μm in the x-y plane and 7.93 μm in the z-direction. The proposed architecture has risen to the challenges posed when imaging microfluidic devices and live processes, as it can successfully acquire 3D volumetric images together with time-lapse recordings, and it is thus a suitable microscopic technique for live tracking miniaturized tissue and disease models.


  • Biology and Biomedicine
  • Electronics
  • Materials science and engineering
  • Mechanical Engineering
  • Naval Engineering
  • Robotics and Industrial Informatics


  • light sheet fluorescence microscopy; live-cell imaging; microfluidic devices