Sound from media increases the immersion of the audience in the story, adding credibilityto the narration but also generating emotions in the spectator. A study on children aged 9-13 years (N = 253), using an audio story, investigated the emotional impact of arousalvs. neutral treatment of sound and 3D vs. stereo mix spatialization. The emotional impactwas measured combining three different measures: physiological (Electrodermal activity),self-report (pre-post exposition), and richness of mentalimages elicited by the story(using Think-aloud technique after exposition). Results showed higher emotional impactof the arousal and 3D audio conditions with different patterns according to the age of theparticipants and distinctive types of interaction when both variables were combined.