A new antibiotic-loaded sol-gel can prevent bacterial prosthetic joint infection: from in vitro studies to an in vivo model Articles uri icon

authors

  • AGUILERA CORREA, JOHN JAIRO
  • GARCIA CASAS, AMAYA
  • MEDIERO, ARANZAZU
  • ROMERA, DAVID
  • MULERO, FRANCISCA
  • CUEVAS LOPEZ, IRENE
  • JIMENEZ MORALES, ANTONIA
  • ESTEBAN, JAIME

publication date

  • January 2020

start page

  • 1

end page

  • 14

issue

  • 2935

volume

  • 10

International Standard Serial Number (ISSN)

  • 1664-302X

abstract

  • The aim of this study was to evaluate the effect of a moxifloxacin-loaded organic-inorganic sol-gel with different antibiotic concentration in the in vitro biofilm development and treatment against Staphylococcus aureus, S. epidermidis, and Escherichia coli, cytotoxicity and cell proliferation of MC3T3-E1 osteoblasts; and its efficacy in preventing the prosthetic joint infection (PJI) caused by clinical strains of S. aureus and E. coli using an in vivo murine model. Three bacterial strains, S. epidermidis ATCC 35984, S. aureus 15981, and, E. coli ATCC 25922, were used for microbiological studies. Biofilm formation was induced using tryptic-soy supplemented with glucose for 24 h, and then, adhered and planktonic bacteria were estimated using drop plate method and absorbance, respectively. A 24-h-mature biofilm of each species growth in a 96-well plate was treated for 24 h using a MBECTM biofilm Incubator lid with pegs coated with the different types of sol-gel, after incubation, biofilm viability was estimated using alamrBlue. MC3T3-E1 cellular cytotoxicity and proliferation were evaluated using CytoTox 96 Non-Radioactive Cytotoxicity Assay and alamarBlue, respectively. The microbiological studies showed that sol-gel coatings inhibited the biofilm development and treated to a mature biofilm of three evaluated bacterial species [...]

keywords

  • escherichia coli; moxifloxacin; prosthetic joint infection; sol-gel; staphylococcus aureus; staphylococcus epidermidis