Impact of optical tissue clearing on the Brillouin signal from biological tissue samples Articles uri icon

publication date

  • June 2019

start page

  • 2674

end page

  • 2683

issue

  • 6

volume

  • 10

international standard serial number (ISSN)

  • 2156-7085

abstract

  • Brillouin spectroscopy is a well-established technology in condensed matter physics to characterize the mechanical properties of inert materials, and it has been extended very recently to the study of biological samples. Transparency is beneficial for samples to be properly analyzed by Brillouin spectroscopy. Here, we explored the efficacy of optical tissue clearing techniques to improve the acquisition of Brillouin spectra from biological tissues in order to analyze their biomechanical properties. We describe the first application of Brillouin scattering to optically cleared biological tissues with CUBIC protocol. We conclude that, within the range of error, tissue clearing does not modify the mechanical properties of the studied biological tissues. (C) 2019 Optical Society of America under the terms of the OSA Open Access Publishing Agreement

keywords

  • confocal brillouin; elastic properties; whole-brain; spectroscopy; scattering; heart