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Herein, we report blue-emitting methoxy-substituted 2, 4-diphenyl quinoline phosphor prepared using acid-catalyzed Friedlander reaction along with the enhancement in the phase purity due to employed thermal treatment in Ar atmosphere. Improvement in the phase purity of the phosphor leading to a change in their optical properties is reported. XRD and FTIR analyses confirm the formation and improvement in the phase purity of the phosphor. TG/DTA analysis endorses the change in their behavior and thermal stability. The modification in optical band gap of the phosphors due to thermal treatment has been confirmed from the UV-vis spectroscopy. Enhancement in the fluorescence intensity of blue-emitting phosphor due to thermal treatment has been described through photoluminescence analysis. Luminescence decay profile has also been recorded using time-correlated single-photon counting (TCSPC) system for the lifetime analysis of the phosphors which supports the obtained PL analysis. The overall study indicates that methoxy-substituted 2, 4-diphenyl quinoline phosphor which was annealed at 90 degrees C in Ar atmosphere serves better fluorescence intensity, thermal stability and lifetime profile, and may be utilized for the organic display applications.