Dynamic Measurement of Tumor Vascular Permeability and Perfusion using a Hybrid System for Simultaneous Magnetic Resonance and Fluorescence Imaging Articles uri icon

authors

  • REN, WUWEI
  • ELMER, ANDREAS
  • BUEHLMANN, DAVID
  • AUGATH, MARK-AUREL
  • VATS, DIVYA
  • RIPOLL LORENZO, JORGE
  • RUDIN, MARKUS

publication date

  • April 2016

start page

  • 191

end page

  • 200

issue

  • 2

volume

  • 18

international standard serial number (ISSN)

  • 1536-1632

electronic international standard serial number (EISSN)

  • 1860-2002

abstract

  • Purpose: Assessing tumor vascular features including permeability and perfusion is essential for diagnostic and therapeutic purposes. The aim of this study was to compare fluorescence and magnetic resonance imaging (MRI)-based vascular readouts in subcutaneously implanted tumors in mice by simultaneous dynamic measurement of tracer uptake using a hybrid fluorescence molecular tomography (FMT)/MRI system. Procedure: Vascular permeability was measured using a mixture of extravascular imaging agents, GdDOTA and the dye Cy5.5, and perfusion using a mixture of intravascular agents, Endorem and a fluorescent probe (Angiosense). Dynamic fluorescence reflectance imaging (dFRI) was integrated into the hybrid system for high temporal resolution. Results: Excellent correspondence between uptake curves of Cy5.5/GdDOTA and Endorem/Angiosense has been found with correlation coefficients R > 0.98. The two modalities revealed good agreement regarding permeability coefficients and centers-of-gravity of the imaging agent distribution. Conclusion: The FMT/dFRI protocol presented is able to accurately map physiological processes and poses an attractive alternative to MRI for characterizing tumor neoangiogenesis.

keywords

  • fluorescence molecular tomography; mri; simultaneous hybrid measurements; tumor angiogenesis; vascular permeability; perfusion; in-vivo; tomography; principles; receptor