A Customized Light Sheet Microscope to Measure Spatio-Temporal Protein Dynamics in Small Model Organisms Articles uri icon

authors

  • RIECKHER, MATTHIAS
  • KYPARISSIDIS-KOKKINIDIS, ILIAS
  • ZACHAROPOULOS, ATHANASIOS
  • KOURMOULAKIS, GEORGIOS
  • TAVERNARAKIS, NEKTARIOS
  • RIPOLL LORENZO, JORGE
  • ZACHARAKIS, GIANNIS

publication date

  • May 2015

start page

  • 1

end page

  • 15

issue

  • 5

volume

  • 10

international standard serial number (ISSN)

  • 1932-6203

abstract

  • We describe a customizable and cost-effective light sheet microscopy (LSM) platform for rapid three-dimensional imaging of protein dynamics in small model organisms. The system is designed for high acquisition speeds and enables extended time-lapse in vivo experiments when using fluorescently labeled specimens. We demonstrate the capability of the setup to monitor gene expression and protein localization during ageing and upon starvation stress in longitudinal studies in individual or small groups of adult Caenorhabditis elegans nematodes. The system is equipped to readily perform fluorescence recovery after photobleaching (FRAP), which allows monitoring protein recovery and distribution under low photobleaching conditions. Our imaging platform is designed to easily switch between light sheet microscopy and optical projection tomography (OPT) modalities. The setup permits monitoring of spatio-temporal expression and localization of ageing biomarkers of subcellular size and can be conveniently adapted to image a wide range of small model organisms and tissue samples.