Fluorescence Lifetime Imaging from Time Resolved Measurements using a Shape-Based Approach Articles uri icon

publication date

  • May 2009

start page

  • 8843

end page

  • 8855

issue

  • 11

volume

  • 17

International Standard Serial Number (ISSN)

  • 1094-4087

abstract

  • We present a novel fluorescent tomography algorithm to estimate the spatial distribution of fluorophores and the fluorescence lifetimes from surface time resolved measurements. The algorithm is a hybridization of the level set
    technique for recovering the distributions of distinct fluorescent markers with
    a gradient method for estimating their lifetimes. This imaging method offers
    several advantages compared to more traditional pixel-based techniques as, for
    example, well defined boundaries and a better resolution of the images. The
    numerical experiments show that our imaging method gives rise to accurate
    reconstructions in the presence of data noise and fluorescence background even
    for complicated fluorophore distributions in several-centimiter-thick biological
    tissue.