Unraveling the aging-reversal potency of stem cell-derived extracellular vesicles in a rat model of premature cardiac senescence Articles uri icon

authors

  • GOMEZ CID, LIDIA
  • CAMPO FONSECA, ALEXIA
  • CERVERA NEGUERUELA, MAR
  • OCAMPO, ALEJANDRA
  • PINTO, ANGEL
  • GIL JAURENA, JUAN MIGUEL
  • SUAREZ SANCHO, SUSANA
  • FERNÁNDEZ AVILÉS, FRANCISCO
  • GRIGORIAN-SHAMAGIAN, LILIAN

publication date

  • July 2025

start page

  • 12910-1

end page

  • 12910-19

issue

  • 112910

volume

  • 28

International Standard Serial Number (ISSN)

  • 2589-0042

abstract

  • Cardiosphere-derived cells (CDCs) and their extracellular vesicles (CDC-EVs) can rejuvenate and improve
    cardiac function, but their efficacy varies among donors. This study aimed to identify predictors of CDC-
    EV potency. CDCs from human donors were analyzed for phenotypical and biological properties. The anti-
    aging activity of CDC-EVs was tested using an in vitro matrix assay. Results showed that donor age did
    not determine potency, but CDC senescence correlated with most bioactive properties. However, senes-
    cence alone was insufficient to predict CDC-EV potency. CDC-EVs were classified as more potent (P-EVs)
    or less potent (NP-EVs) based on assay performance. In a rat model of premature cardiac aging, P-EVs
    reduced senescence-associated GLB1 gene expression and protected against hypertrophy and fibrosis,
    while NP-EVs had negative effects. The study concludes that CDC-EV anti-aging potency cannot be pre-
    dicted by donor age or CDC senescence, proposing an in vitro potency assay for evaluating CDC-EVs before
    clinical use.

subjects

  • Biology and Biomedicine