The local microenvironment drives activation of neutrophils in human brain tumors Articles uri icon

authors

  • MAAS, ROELTJE R.
  • SOUKUP, KLARA
  • FOURNIER, NADINE
  • MASSARA, MATTEO
  • GALLAND, SABINE
  • KORNETE, MARA
  • WISCHNEWSKI, VLADIMIR
  • LOURENCO, JOAO
  • CROCI, DAVIDE
  • ÁLVAREZ-PRADO, ÁNGEL F.
  • MARIE, DAMIEN N.
  • LILJA, JOHANNA
  • MARCONE, RACHEL
  • CALVO, GABRIEL F.
  • SANTALLA MENDEZ, RUI
  • AUBEL, PAULINE
  • BEJARANO, LEIRE
  • WIRAPATI, PRATYAKSHA
  • BALLESTEROS MARIN, IVAN JOSE
  • HIDALGO, ANDRÉS
  • HOTTINGER, ANDREAS F.
  • BROULAND, JEAN-PHILIPPE
  • DANIEL, ROY T.
  • HEGI, MONIKA E.
  • JOYCE, JOHANNA A.

published in

publication date

  • October 2023

start page

  • 4546

end page

  • 4566.e27

issue

  • 21

volume

  • 186

International Standard Serial Number (ISSN)

  • 0092-8674

Electronic International Standard Serial Number (EISSN)

  • 1097-4172

abstract

  • Neutrophils are abundant immune cells in the circulation and frequently infiltrate tumors in substantial numbers. However, their precise functions in different cancer types remain incompletely understood, including in the brain microenvironment. We therefore investigated neutrophils in tumor tissue of glioma and brain metastasis patients, with matched peripheral blood, and herein describe the first in-depth analysis of neutrophil phenotypes and functions in these tissues. Orthogonal profiling strategies in humans and mice revealed that brain tumor-associated neutrophils (TANs) differ significantly from blood neutrophils and have a prolonged lifespan and immune-suppressive and pro-angiogenic capacity. TANs exhibit a distinct inflammatory signature, driven by a combination of soluble inflammatory mediators including tumor necrosis factor alpha (TNF-alpha) and Ceruloplasmin, which is more pronounced in TANs from brain metastasis versus glioma. Myeloid cells, including tumor-associated macrophages, emerge at the core of this network of pro-inflammatory mediators, supporting the concept of a critical myeloid niche regulating overall immune suppression in human brain tumors.

subjects

  • Biology and Biomedicine

keywords

  • tumor microenvironment; tumor immune microenvironment; glioma; glioblastoma; brain metastasis; tumor immunology; neutrophil-to-lymphocyte ratio; immune suppression; angiogenesis; myeloid niche; tnf-alpha