Analyses of H2O2 production in time during mechanical-like injury Articles uri icon

publication date

  • November 2023

start page

  • S34

volume

  • 208, Supplement 1

International Standard Serial Number (ISSN)

  • 0891-5849

abstract

  • The mechanisms that regulate skin physiology still remain relatively poorly understood. This impacts, even more, our comprehension of skin diseases which are one of the primary causes of global non-fatal disorders. Hence, understanding better the physiology that governs keratinocytes differentiation is an essential unmet medical need. At the same time, the field of redox biology arises, more and more, as a central modulator of many intracellular pathways. Specifically, H2O2 -the main signaling molecule in redox biology- acts as a vital second messenger. Although a role for H2O2 in cell migration and tissue regeneration in the skin has been already observed in animal models like zebrafish, redox signaling in these processes in humans remains mostly unexplored. To start solving this intriguing question, we decided to use an immortalized keratinocytic cell line to characterize the redox intracellular environment upon a mechanical-like injury. Thus, we engineered the cells with the H2O2-sensitive fluorescent HyPer7 in the cytosol for monitoring H2O2-fluctuation during proliferation and migration in live imaging. The obtained data revealed that shifts in the cytosolic H2O2 levels could be detected during the gap repair process and provided insights into distinct cell behaviors. To sum up, these results make the first move in the comprehension of H2O2's importance in human keratinocytes, suggesting the presence of an intricate interplay of ROS within cellular processes.

subjects

  • Biology and Biomedicine