In this work, we report an electrochemical cholesterol biosensor based on cholesterol oxidase(ChOx) enzyme immobilized on TiO2- nanoparticles – reduced graphene oxide(rGO) – polypyrrole (PPy) nanocomposite modified electrode.The electrochemical properties of GCE modified PPy (PPy-GCE) were studied using CV (Cyclic Voltammetry) and DPV (Differential Pulse Voltammetry). The developed sensor exhibited piecewise linearity from 0.1 μM to 1 μM and from 1 μM to 600 μM with the sensitivity of 61.665 and 0.1466 (2 mA mM × cm) respectively. The limit of detection of the sensor was found to be 32 nm.
Classification
subjects
Chemistry
Materials science and engineering
keywords
chemical oxidative polymerization; clinical analysis; nanomaterials; non-enzymatic