Transfer of H2O2 from Mitochondria to the endoplasmic reticulum via Aquaporin-11 Articles uri icon

publication date

  • September 2022

start page

  • 1

end page

  • 8


  • 102410


  • 55

International Standard Serial Number (ISSN)

  • 2213-2317


  • Some aquaporins (AQPs) can transport H2O2 across membranes, allowing redox signals to proceed in and between cells. Unlike other peroxiporins, human AQP11 is an endoplasmic reticulum (ER)-resident that can conduit H2O2 to the cytosol. Here, we show that silencing Ero1α, an ER flavoenzyme that generates abundant H2O2 during oxidative folding, causes a paradoxical increase in luminal H2O2 levels. The simultaneous AQP11 downregulation prevents this increase, implying that H2O2 reaches the ER from an external source(s). Pharmacological inhibition of the electron transport chain reveals that Ero1α downregulation activates superoxide production by complex III. In the intermembrane space, superoxide dismutase 1 generates H2O2 that enters the ER channeled by AQP11. Meanwhile, the number of ER-mitochondria contact sites increases as well, irrespective of AQP11 expression. Taken together, our findings identify a novel interorganellar redox response that is activated upon Ero1α downregulation and transfers H2O2 from mitochondria to the ER via AQP11.


  • Biology and Biomedicine


  • hydrogen peroxide; redox homeostasis; interorganellar crosstalk/ peroxiporin; complex iii; mitochondrial-associated membranes