Development of an intracellular screen for new compounds able to inhibit Mycobacterium tuberculosis growth in human macrophages Articles uri icon

authors

  • SORRENTINO, FLAVIA
  • GONZÁLEZ DEL RÍO, RUBÉN
  • ZHENG, XINGJI
  • PRESA MATILLA, JESÚS
  • TORRES GÓMEZ, PEDRO
  • MARTÍNEZ HOYOS, MARÍA
  • PÉREZ HERRÁN, MARÍA ESTHER
  • AV-GAY, YOSSEF
  • MENDOZA LOSANA, ALFONSO

publication date

  • October 2015

start page

  • 640

end page

  • 645

issue

  • 1

volume

  • 60

International Standard Serial Number (ISSN)

  • 0066-4804

Electronic International Standard Serial Number (EISSN)

  • 1098-6596

abstract

  • Here we describe the development and validation of an intracellular high-throughput screening assay for finding new antituberculosis compounds active in human macrophages. The assay consists of a luciferase-based primary identification assay, followed by a green fluorescent protein-based secondary profiling assay. Standard tuberculosis drugs and 158 previously recognized active antimycobacterial compounds were used to evaluate assay robustness. Data show that the assay developed is a short and valuable tool for the discovery of new antimycobacterial compounds.