Proteomics of multigenic families from species under represented in databases: The case of loquat (eriobotrya japonica lindl.) polyphenol oxidases

Here, we approach the problem of obtaining accurate and reliable information about the gene origin of a protein belonging to a multigenic family, polyphenol oxidase, from an underrepresented species, Eriobotrya japonica. De novo sequencing was a key approach to obtain broad sequence coverage. Alignment of peptides on their most similar homologous protein revealed divergent amino acid positions that lead to hypothesize the minimal number of genes encoding for the proteins analyzed. © 2008 American Chemical Society.

de novo sequencing multigenic families phylogenetic analysis polyphenol oxidase underrepresented species in databases catechol oxidase catechol oxidase amino terminal sequence article controlled study loquat nonhuman phylogeny priority journal protein analysis proteomics sequence homology amino acid sequence antibody production chemistry enzymology genetics metabolism molecular genetics multigene family polyacrylamide gel electrophoresis protein database solubility western blotting eriobotrya japonica amino acid sequence antibody formation blotting; western catechol oxidase databases; protein electrophoresis; polyacrylamide gel eriobotrya molecular sequence data multigene family phylogeny sequence homology; amino acid solubility

Proteomics of multigenic families from species under represented in databases: The case of loquat (eriobotrya japonica lindl.) polyphenol oxidases Articles