New Strategies for Echocardiographic Evaluation of Left Ventricular Function in a Mouse Model of Long-Term Myocardial Infarction

Abstract: Background: The aim of this article is to present an optimized acquisition and analysis protocol for the echocardiographic evaluation of left ventricle (LV) remodeling in a mouse model of myocardial infarction (MI). Methodology: 13 female DBA/2J mice underwent permanent occlusion of the left anterior descending (LAD) coronary artery leading to MI. Mice echocardiography was performed using a Vevo 770 (Visualsonics, Canada) before infarction, and 7, 14, 30, 60, 90 and 120 days after LAD ligation. LV systolic function was evaluated using different parameters, including the fractional area change (FAC%) computed in four high-temporal resolution B-mode short axis images taken at different ventricular levels, and in one parasternal long axis. Pulsed wave and tissue Doppler modes were used to evaluate the diastolic function and Tei Index for global cardiac function. The echocardiographic measurements of infarct size were validated histologically using collagen deposition labeled by Sirius red staining. All data was analyzed using Shapiro-Wilk and Student's t-tests.

animal experiment; animal model; animal tissue; article; b scan; cardiac imaging; controlled study; echocardiography; female; functional assessment; functional morphology; heart infarction; heart infarction size; heart left ventricle ejection fraction; heart left ventricle enddiastolic volume; heart left ventricle function; left ventricular systolic dysfunction; mouse; nonhuman; pulse wave; quantitative analysis; animals; disease models; animal; echocardiography; image processing; computer assisted; mice; myocardial infarction; time factors; ventricular function; left; ventricular remodeling; mus

New Strategies for Echocardiographic Evaluation of Left Ventricular Function in a Mouse Model of Long-Term Myocardial Infarction Articles