3D Reconstruction of Histological Sections: Applicationto Mammary Gland Tissue Articles uri icon

publication date

  • October 2010

start page

  • 1019

end page

  • 1029


  • 11


  • 73

International Standard Serial Number (ISSN)

  • 1059-910X

Electronic International Standard Serial Number (EISSN)

  • 1097-0029


  • In this article, we present a novel method for the automatic 3D reconstruction ofthick tissue blocks from 2D histological sections. The algorithm completes a high-content (multi-scale, multifeature) imaging system for simultaneous morphological and molecular analysis ofthick tissue samples. This computer-based system integrates image acquisition, annotation, regis-tration, and three-dimensional reconstruction. We present an experimental validation of this toolusing both synthetic and real data. In particular, we present the 3D reconstruction of an entiremouse mammary gland and demonstrate the integration of high-resolution molecular data.


  • computer assisted microscopy; assisted histopathology; image registration; paraffin; algorithm; animal; article; breast; computer graphics; cytochemistry; female; histology; image processing; image quality; methodology; microphotography; microscopy; mouse; reproducibility; thorax; algorithms; animals; histocytochemistry; mice; paraffin embedding; phantoms; imaging; photomicrography; reproducibility of results