Photoactivatable Caged Cyclic RGD Peptide for Triggering Integrin Binding and Cell Adhesion to Surfaces Articles uri icon

authors

  • Wirkner, Melanie
  • Weis, Simone
  • SAN MIGUEL ARNANZ, VERONICA
  • Álvarez, Marta
  • Gropeanu, Radu A.
  • Salierno, Marcelo
  • Sartoris, Anne
  • Unger, Ronald E.
  • Kirkpatrick, James
  • del Campo, Aránzazu

publication date

  • November 2011

start page

  • 2623

end page

  • 2629

issue

  • 17

volume

  • 12

International Standard Serial Number (ISSN)

  • 1439-4227

Electronic International Standard Serial Number (EISSN)

  • 1439-7633

abstract

  • We report the synthesis and properties of a photoactivatable caged RGD peptide and its application for phototriggering integrin- and cell-binding to surfaces. We analysed in detail 1) the differences in the integrin-binding affinity of the caged and uncaged forms by quartz crystal microbalance (QCM) studies, 2) the efficiency and yield of the photolytic uncaging reaction, 3) the biocompatibility of the photolysis by-products and irradiation conditions, 4) the possibility of site, temporal and density control of integrin-binding and therefore human cell attachment, and 5) the possibility of in situ generation of cell patterns and cell gradients by controlling the UV exposure. These studies provide a clear picture of the potential and limitations of caged RGD for integrin-mediated cell adhesion and demonstrate the application of this approach to the control and study of cell interactions and responses.

subjects

  • Chemistry
  • Materials science and engineering