3D bioprinting of functional human skin: production and in vivo analysis Articles uri icon

publication date

  • December 2016

start page

  • 1

end page

  • 2

issue

  • 1 (015006)

volume

  • 9

International Standard Serial Number (ISSN)

  • 1758-5082

Electronic International Standard Serial Number (EISSN)

  • 1758-5090

abstract

  • Significant progress has been made over the past 25 years in the development of in vitro-engineered substitutes that mimic human skin, either to be used as grafts for the replacement of lost skin, or for the establishment of in vitro human skin models. In this sense, laboratory-grown skin substitutes containing dermal and epidermal components offer a promising approach to skin engineering. In particular, a human plasma-based bilayered skin generated by our group, has been applied successfully to treat burns as well as traumatic and surgical wounds in a large number of patients in Spain. There are some aspects requiring improvements in the production process of this skin; for example, the relatively long time (three weeks) needed to produce the surface required to cover an extensive burn or a large wound, and the necessity to automatize and standardize a process currently performed manually.

keywords

  • 3d bioprinting; skin bioprinting; artificial skin; skin equivalents; skin tissue engineering; 3d skin culture; fibrin hydrogel; cultured epithelial autograft; human epidermal-keratinocytes; tissue-engineered skin; stem-cells; bioengineered skin; drug-delivery; burn injuries; skin substitutes; differentiation; regeneration