IKK alpha regulates the stratification and differentiation of the epidermis: implications for skin cancer development Articles uri icon

authors

  • ALAMEDA, JOSEFA P.
  • FERNANDEZ ACENERO, MARIA JESUS
  • BRAVO, ANA
  • DE LOS LLANOS CASANOVA, MARIA
  • NAVARRO, MANUEL
  • RAMIREZ, ANGEL
  • PAGE, ANGUSTIAS
  • SUAREZ CABRERA, CRISTIAN
  • MORENO MALDONADO, RODOLFO
  • PARAMIO, JESUS M.
  • DEL CARMEN FARINA, MARIA
  • RIO NECHAEVSKY, MARCELA ANDREA DEL

publication date

  • November 2016

start page

  • 76779

end page

  • 76792

issue

  • 47

volume

  • 7

international standard serial number (ISSN)

  • 1949-2553

abstract

  • IKK alpha plays a mandatory role in keratinocyte differentiation and exerts an important task in non-melanoma skin cancer development. However, it is not fully understood how IKK alpha exerts these functions. To analyze in detail the role of IKK alpha in epidermal stratification and differentiation, we have generated tridimensional (3D) cultures of human HaCaT keratinocytes and fibroblasts in fibrin gels, obtaining human skin equivalents that comprise an epidermal and a dermal compartments that resembles both the structure and differentiation of normal human skin. We have found that IKK alpha expression must be strictly regulated in epidermis, as alterations in its levels lead to histological defects and promote the development of malignant features.

keywords

  • ikk alpha; keratinocyte differentiation; mmp9; skin; skin cancer, nf-kappa-b; keratinocyte differentiation; enrichment analysis; mice lacking; expression; kinase; activation; progression; metastasis; carcinomas