In this study the first results on evaluation and assessment of grafted bioengineered skin substitutes using an optical Diffuse Reflectance Spectroscopy (DRS) system with a remote optical probe are shown. The proposed system is able to detect early vascularization of skin substitutes expressing the Vascular Endothelial Growth Factor (VEGF) protein compared to normal grafts, even though devitalized skin is used to protect the grafts. Given the particularities of the biological problem, data analysis is performed using two Blind Signal Separation (BSS) methods: Principal Component Analysis (PCA) and Independent Component Analysis (ICA). These preliminary results are the first step towards point-of-care diagnostics for skin implants early assessment.
blind source separation; endothelial cells; independent component analysis; reflection; spectroscopy; tissue engineering; biological problems; blind signal separation; diffuse reflectance spectroscopy; evaluation and assessment; independent component analysis (ICA); point of care diagnostic; skin substitutes; vascular endothelial growth factor; principal component analysis; vasculotropin; animal experiment; animal tissue; article; biopsy; diffuse reflectance pectroscopy; histology; independent component analysis; monte carlo method; mouse; nonhuman; principal component analysis; protein expression; tissue engineering